› 5cfy

X-ray diffraction
3.5Å resolution

CRYSTAL STRUCTURE OF GLTPH R397A IN COMPLEX WITH NA+ AND L-ASP

Released:
DOI: 10.2210/pdb5cfy/pdb
PDB entry 5cfy coloured by chain, front view.
PDB entry 5cfy coloured by chain, side view.
PDB entry 5cfy coloured by chain, top view.
Source organism: Pyrococcus horikoshii OT3
Primary publication:
Coupled ion binding and structural transitions along the transport cycle of glutamate transporters.
Verdon G, Oh S, Serio RN, Boudker O
Elife 3 e02283 (2014)
PMID: 24842876

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-129795 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glutamate transporter homolog Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 425 amino acids
Theoretical weight: 44.82 KDa
Source organism: Pyrococcus horikoshii OT3
Expression system: Escherichia coli
UniProt:
  • Canonical: O59010 (Residues: 1-425; Coverage: 100%)
Gene name: PH1295
Sequence domains: Sodium:dicarboxylate symporter family
Structure domains: Sodium:dicarboxylate symporter

Ligands and Environments

2 bound ligands:
Ligand ASP 6 x ASP
Ligand NA 12 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P31
Unit cell:
a: 116.958Å b: 116.958Å c: 313.517Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.251 0.249 0.294
Expression system: Escherichia coli

Quick links

Citations

11 review citations

Shared Molecular Mechanisms of Membrane Transporters.
Drew et al. (2016)
10 more

3 mentions without citation

Elevator-type mechanisms of membrane transport.
Garaeva et al. (2020)
2 more