› 5apr

X-ray diffraction
2.1Å resolution

STRUCTURES OF COMPLEXES OF RHIZOPUSPEPSIN WITH PEPSTATIN AND OTHER STATINE-CONTAINING INHIBITORS

Released:
DOI: 10.2210/pdb5apr/pdb
PDB entry 5apr coloured by chain, front view.
PDB entry 5apr coloured by chain, side view.
PDB entry 5apr coloured by chain, top view.
Source organism: Rhizopus microsporus var. chinensis
Primary publication:
Structures of complexes of rhizopuspepsin with pepstatin and other statine-containing inhibitors.
Suguna K, Padlan EA, Bott R, Boger J, Parris KD, Davies DR
Proteins 13 195-205 (1992)
PMID: 1603809

Function and Biology Details

Reaction catalysed: 
Hydrolysis of proteins with broad specificity similar to that of pepsin A, preferring hydrophobic residues at P1 and P1'. Clots milk and activates trypsinogen. Does not cleave 4-Gln-|-His-5, but does cleave 10-His-|-Leu-11 and 12-Val-|-Glu-13 in B chain of insulin.
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-138858 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Rhizopuspepsin Chain: E
Molecule details ›
Chain: E
Length: 325 amino acids
Theoretical weight: 34.07 KDa
Source organism: Rhizopus microsporus var. chinensis
Expression system: Not provided
UniProt:
  • Canonical: P06026 (Residues: 69-393; Coverage: 87%)
Sequence domains: Eukaryotic aspartyl protease
Structure domains: Acid Proteases
PEPSTATIN-LIKE RENIN INHIBITOR Chain: I
Molecule details ›
Chain: I
Length: 8 amino acids
Theoretical weight: 980 Da
Source organism: Rhizopus microsporus var. chinensis
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand CA 1 x CA
1 modified residue:
Ligand DHL 1 x DHL

Experiments and Validation Details

Entry percentile scores
Spacegroup: P212121
Unit cell:
a: 60.33Å b: 60.64Å c: 106.76Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.144 not available not available
Expression system: Not provided

Quick links

Citations

2 review citations

Inhibitors of aspartyl proteinases.
Abdel-Meguid SS. (1993)
1 more

3 mentions without citation

Analysis of crystal structures of aspartic proteinases: on the role of amino acid residues adjacent to the catalytic site of pepsin-like enzymes.
Andreeva et al. (2001)
2 more