› 5am8

X-ray diffraction
1.9Å resolution

Crystal structure of the Angiotensin-1 converting enzyme N-domain in complex with amyloid-beta 4-10

Released:
DOI: 10.2210/pdb5am8/pdb
PDB entry 5am8 coloured by chain, front view.
PDB entry 5am8 coloured by chain, side view.
PDB entry 5am8 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Kinetic and structural characterization of amyloid-β peptide hydrolysis by human angiotensin-1-converting enzyme.
Larmuth KM, Masuyer G, Douglas RG, Schwager SL, Acharya KR, Sturrock ED
FEBS J 283 1060-76 (2016)
PMID: 26748546

Function and Biology Details

Reaction catalysed: 
Release of a C-terminal dipeptide, oligopeptide-|-Xaa-Yaa, when Xaa is not Pro, and Yaa is neither Asp nor Glu. Thus, conversion of angiotensin I to angiotensin II, with increase in vasoconstrictor activity, but no action on angiotensin II.
Biochemical function:
Biological process:
Cellular component:
Sequence domain:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-138357 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (7 distinct):
Angiotensin-converting enzyme, soluble form Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 629 amino acids
Theoretical weight: 72.59 KDa
Source organism: Homo sapiens
Expression system: Cricetulus griseus
UniProt:
  • Canonical: P12821 (Residues: 30-658; Coverage: 49%)
Gene names: ACE, DCP, DCP1
Sequence domains: Angiotensin-converting enzyme
Amyloid-beta protein 40 Chains: P, Q, R, S
Molecule details ›
Chains: P, Q, R, S
Length: 7 amino acids
Theoretical weight: 883 Da
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P05067 (Residues: 675-681; Coverage: 1%)
Gene names: A4, AD1, APP

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, FUC
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA, FUL
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, FUC, MAN
Carbohydrate polymer
6 bound ligands:
Ligand ZN 4 x ZN
Ligand CL 4 x CL
Ligand SO4 4 x SO4
Ligand NAG 3 x NAG
Ligand PEG 18 x PEG
Ligand P6G 3 x P6G
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: P1
Unit cell:
a: 73.449Å b: 101.76Å c: 114.361Å
α: 85.23° β: 86.07° γ: 81.45°
R-values:
R R work R free
0.187 0.185 0.224
Expression systems:
  • Cricetulus griseus
  • Not provided

Quick links

Citations

2 review citations

ACE2 and ACE: structure-based insights into mechanism, regulation and receptor recognition by SARS-CoV.
Lubbe et al. (2020)
1 more

1 mention without citation

Kinetic and structural characterization of amyloid-β peptide hydrolysis by human angiotensin-1-converting enzyme.
Larmuth et al. (2016)