› 5akd

X-ray diffraction
7.6Å resolution

MutS in complex with the N-terminal domain of MutL - crystal form 3

Released:
DOI: 10.2210/pdb5akd/pdb
PDB entry 5akd coloured by chain, front view.
PDB entry 5akd coloured by chain, side view.
PDB entry 5akd coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
MutS/MutL crystal structure reveals that the MutS sliding clamp loads MutL onto DNA.
Groothuizen FS, Winkler I, Cristóvão M, Fish A, Winterwerp HH, Reumer A, Marx AD, Hermans N, Nicholls RA, Murshudov GN, Lebbink JH, Friedhoff P, Sixma TK
Elife 4 e06744 (2015)
PMID: 26163658

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-149935 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
DNA mismatch repair protein MutS Chains: A, B, E, F, I, J
Molecule details ›
Chains: A, B, E, F, I, J
Length: 800 amino acids
Theoretical weight: 89.48 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli B
UniProt:
  • Canonical: P23909 (Residues: 1-800; Coverage: 94%)
Gene names: JW2703, b2733, fdv, mutS
Sequence domains:
DNA mismatch repair protein MutL Chains: C, D, G, H, K, L
Molecule details ›
Chains: C, D, G, H, K, L
Length: 369 amino acids
Theoretical weight: 41.28 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P23367 (Residues: 1-349; Coverage: 57%)
Gene names: JW4128, b4170, mutL
Sequence domains:

Ligands and Environments

1 bound ligand:
Ligand ANP 6 x ANP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06DA
Spacegroup: P21
Unit cell:
a: 193.02Å b: 109.762Å c: 275.841Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.266 0.264 0.306
Expression systems:
  • Escherichia coli B
  • Escherichia coli BL21(DE3)

Quick links

Citations

10 review citations

Invited review: Architectures and mechanisms of ATP binding cassette proteins.
Hopfner KP. (2016)
9 more

1 mention without citation

MutS/MutL crystal structure reveals that the MutS sliding clamp loads MutL onto DNA.
Groothuizen et al. (2015)