› 5aga

X-ray diffraction
2.9Å resolution

Crystal structure of the Helicase domain of human DNA polymerase theta in complex with AMPPNP

Released:
DOI: 10.2210/pdb5aga/pdb
PDB entry 5aga coloured by chain, front view.
PDB entry 5aga coloured by chain, side view.
PDB entry 5aga coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure of the Helicase Domain of DNA Polymerase Theta Reveals a Possible Role in the Microhomology-Mediated End-Joining Pathway.
Newman JA, Cooper CDO, Aitkenhead H, Gileadi O
Structure 23 2319-2330 (2015)
PMID: 26636256

Function and Biology Details

Reactions catalysed: 
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)
ATP + H(2)O = ADP + phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
homo tetramer
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-130953 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DNA polymerase theta Chain: A
Molecule details ›
Chain: A
Length: 830 amino acids
Theoretical weight: 93.2 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: O75417 (Residues: 67-894; Coverage: 32%)
Gene names: POLH, POLQ
Sequence domains:

Ligands and Environments

4 bound ligands:
Ligand ANP 1 x ANP
Ligand FLC 1 x FLC
Ligand K 1 x K
Ligand MG 1 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04-1
Spacegroup: I222
Unit cell:
a: 116.17Å b: 132.71Å c: 156.43Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.22 0.217 0.264
Expression system: Spodoptera frugiperda

Quick links

Citations

20 review citations

DNA polymerase θ (POLQ), double-strand break repair, and cancer.
Wood et al. (2016)
19 more

8 mentions without citation

DNA Polymerase θ: A Unique Multifunctional End-Joining Machine.
Black et al. (2016)
7 more