› 5a9q

Electron Microscopy
23Å resolution

Human nuclear pore complex

Released:
DOI: 10.2210/pdb5a9q/pdb
PDB entry 5a9q coloured by chain, front view.
PDB entry 5a9q coloured by chain, side view.
PDB entry 5a9q coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
In situ structural analysis of the human nuclear pore complex.
von Appen A, Kosinski J, Sparks L, Ori A, DiGuilio AL, Vollmer B, Mackmull MT, Banterle N, Parca L, Kastritis P, Buczak K, Mosalaganti S, Hagen W, Andres-Pons A, Lemke EA, Bork P, Antonin W, Glavy JS, Bui KH, Beck M
Nature 526 140-143 (2015)
PMID: 26416747
Related structures: EMD-3103

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero 304-mer (preferred)
PDBe Complex ID:
PDB-CPX-131100 (preferred)
Entry contents:
10 distinct polypeptide molecules
Macromolecules (10 distinct):
Nucleoporin Nup43 Chains: 0, 9, I, R
Molecule details ›
Chains: 0, 9, I, R
Length: 380 amino acids
Theoretical weight: 42.2 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: Q8NFH3 (Residues: 1-380; Coverage: 100%)
Gene name: NUP43
Sequence domains: WD domain, G-beta repeat
Nuclear pore complex protein Nup160 Chains: 1, J, S, a
Molecule details ›
Chains: 1, J, S, a
Length: 1436 amino acids
Theoretical weight: 162.28 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: Q12769 (Residues: 1-1436; Coverage: 100%)
Gene names: KIAA0197, NUP120, NUP160
Sequence domains: Nucleoporin Nup120/160
Nucleoporin Nup37 Chains: 2, K, T, b
Molecule details ›
Chains: 2, K, T, b
Length: 326 amino acids
Theoretical weight: 36.75 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: Q8NFH4 (Residues: 1-326; Coverage: 100%)
Gene name: NUP37
Sequence domains: WD domain, G-beta repeat
Nuclear pore complex protein Nup133 Chains: 3, C, L, U
Molecule details ›
Chains: 3, C, L, U
Length: 1156 amino acids
Theoretical weight: 129.11 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: Q8WUM0 (Residues: 1-1156; Coverage: 100%)
Gene name: NUP133
Sequence domains: Non-repetitive/WGA-negative nucleoporin C-terminal
Nuclear pore complex protein Nup107 Chains: 4, D, M, V
Molecule details ›
Chains: 4, D, M, V
Length: 925 amino acids
Theoretical weight: 106.5 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P57740 (Residues: 1-925; Coverage: 100%)
Gene name: NUP107
Sequence domains: Nuclear pore protein 84 / 107
Nuclear pore complex protein Nup96 Chains: 5, E, N, W
Molecule details ›
Chains: 5, E, N, W
Length: 937 amino acids
Theoretical weight: 106.04 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P52948 (Residues: 881-1817; Coverage: 52%)
Gene names: ADAR2, NUP98
Sequence domains: Nuclear protein 96
Protein SEC13 homolog Chains: 6, F, O, X
Molecule details ›
Chains: 6, F, O, X
Length: 322 amino acids
Theoretical weight: 35.58 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P55735 (Residues: 1-322; Coverage: 100%)
Gene names: D3S1231E, SEC13, SEC13A, SEC13L1, SEC13R
Sequence domains: WD domain, G-beta repeat
Nucleoporin SEH1 Chains: 7, G, P, Y
Molecule details ›
Chains: 7, G, P, Y
Length: 360 amino acids
Theoretical weight: 39.7 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: Q96EE3 (Residues: 1-360; Coverage: 100%)
Gene names: SEC13L, SEH1, SEH1L
Sequence domains: WD domain, G-beta repeat
Nuclear pore complex protein Nup85 Chains: 8, H, Q, Z
Molecule details ›
Chains: 8, H, Q, Z
Length: 656 amino acids
Theoretical weight: 75.11 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: Q9BW27 (Residues: 1-656; Coverage: 100%)
Gene names: NUP75, NUP85, PCNT1
Sequence domains: Nup85 Nucleoporin
Nuclear pore complex protein Nup155 Chains: A, B
Molecule details ›
Chains: A, B
Length: 1391 amino acids
Theoretical weight: 155.36 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: O75694 (Residues: 1-1391; Coverage: 100%)
Gene names: KIAA0791, NUP155
Sequence domains: Nup133 N terminal like

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 23Å
Relevant EMDB volumes: EMD-3103

Quick links

Citations

61 review citations

The Nuclear Pore Complex as a Flexible and Dynamic Gate.
Knockenhauer et al. (2016)
60 more

16 mentions without citation

The Structure Inventory of the Nuclear Pore Complex.
Schwartz TU. (2016)
15 more