› 5a7t

X-ray diffraction
2.4Å resolution

Crystal structure of Sulfolobus acidocaldarius Trm10 at 2.4 angstrom resolution.

Released:
DOI: 10.2210/pdb5a7t/pdb
PDB entry 5a7t coloured by chain, front view.
PDB entry 5a7t coloured by chain, side view.
PDB entry 5a7t coloured by chain, top view.
Source organism: Sulfolobus acidocaldarius
Primary publication:
Structural and functional insights into tRNA binding and adenosine N1-methylation by an archaeal Trm10 homologue.
Van Laer B, Roovers M, Wauters L, Kasprzak JM, Dyzma M, Deyaert E, Kumar Singh R, Feller A, Bujnicki JM, Droogmans L, Versées W
Nucleic Acids Res 44 940-53 (2016)
PMID: 26673726

Function and Biology Details

Reaction catalysed: 
S-adenosyl-L-methionine + adenine(9) in tRNA = S-adenosyl-L-homocysteine + N(1)-methyladenine(9) in tRNA
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-175553 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
tRNA (adenine(9)-N1)-methyltransferase Chain: A
Molecule details ›
Chain: A
Length: 269 amino acids
Theoretical weight: 30.53 KDa
Source organism: Sulfolobus acidocaldarius
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q4J894 (Residues: 1-249; Coverage: 85%)
Gene name: Saci_1677

Ligands and Environments

1 bound ligand:
Ligand PEG 1 x PEG
1 modified residue:
Ligand MSE 5 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: SOLEIL BEAMLINE PROXIMA 1
Spacegroup: P212121
Unit cell:
a: 41.73Å b: 46.4Å c: 139.04Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.182 0.179 0.25
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

8 review citations

The Diversity of Ribonuclease P: Protein and RNA Catalysts with Analogous Biological Functions.
Klemm et al. (2016)
7 more

2 mentions without citation

m1A Post-Transcriptional Modification in tRNAs.
Oerum et al. (2017)
1 more