› 5a6m

X-ray diffraction
1.17Å resolution

Determining the specificities of the catalytic site from the very high resolution structure of the thermostable glucuronoxylan endo-Beta-1, 4-xylanase, CtXyn30A, from Clostridium thermocellum with a xylotetraose bound

Released:
DOI: 10.2210/pdb5a6m/pdb
PDB entry 5a6m coloured by chain, front view.
PDB entry 5a6m coloured by chain, side view.
PDB entry 5a6m coloured by chain, top view.
Source organism: Acetivibrio thermocellus ATCC 27405
Primary publication:
Conservation in the mechanism of glucuronoxylan hydrolysis revealed by the structure of glucuronoxylan xylanohydrolase (CtXyn30A) from Clostridium thermocellum.
Freire F, Verma A, Bule P, Alves VD, Fontes CM, Goyal A, Najmudin S
Acta Crystallogr D Struct Biol 72 1162-1173 (2016)
PMID: 27841749

Function and Biology Details

Reaction catalysed: 
Endohydrolysis of (1->4)-beta-D-xylosidic linkages in xylans
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-107089 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (3 distinct):
Carbohydrate binding family 6 Chain: A
Molecule details ›
Chain: A
Length: 409 amino acids
Theoretical weight: 46.4 KDa
Source organism: Acetivibrio thermocellus ATCC 27405
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: A3DJS9 (Residues: 34-419; Coverage: 61%)
Gene name: Cthe_3012
Sequence domains: Glycosyl hydrolase family 30 beta sandwich domain
Structure domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: XYS, XYP
Carbohydrate polymer
Carbohydrate polymer : NEW Components: XYP
Carbohydrate polymer
2 bound ligands:
Ligand PO4 5 x PO4
Ligand PEG 1 x PEG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SOLEIL BEAMLINE PROXIMA 1
Spacegroup: P21
Unit cell:
a: 49.68Å b: 87.12Å c: 59Å
α: 90° β: 113.36° γ: 90°
R-values:
R R work R free
0.116 0.115 0.135
Expression system: Escherichia coli BL21

Quick links

Citations

3 citation in other articles

A novel fungal GH30 xylanase with xylobiohydrolase auxiliary activity.
Katsimpouras et al. (2019)
2 more

2 mentions without citation

Mode of Action of GH30-7 Reducing-End Xylose-Releasing Exoxylanase A (Xyn30A) from the Filamentous Fungus Talaromyces cellulolyticus.
Nakamichi et al. (2019)
1 more