› 5zof

X-ray diffraction
2.25Å resolution

Crystal Structure of D181A/R192F hFen1 in complex with DNA

Released:
DOI: 10.2210/pdb5zof/pdb
PDB entry 5zof coloured by chain, front view.
PDB entry 5zof coloured by chain, side view.
PDB entry 5zof coloured by chain, top view.
Source organisms:
Primary publication:
Structural basis of 5' flap recognition and protein-protein interactions of human flap endonuclease 1.
Xu H, Shi R, Han W, Cheng J, Xu X, Cheng K, Wang L, Tian B, Zheng L, Shen B, Hua Y, Zhao Y
Nucleic Acids Res 46 11315-11325 (2018)
PMID: 30295841

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-119526 (preferred)
Entry contents:
1 distinct polypeptide molecule
3 distinct DNA molecules
Macromolecules (4 distinct):
Flap endonuclease 1 Chain: A
Molecule details ›
Chain: A
Length: 333 amino acids
Theoretical weight: 37.61 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P39748 (Residues: 1-333; Coverage: 88%)
Gene names: FEN1, RAD2
Sequence domains:
DNA (5'-D(*CP*CP*TP*CP*TP*GP*CP*CP*TP*CP*AP*AP*GP*AP*CP*GP*GP*G)-3') Chain: B
Molecule details ›
Chain: B
Length: 18 nucleotides
Theoretical weight: 5.48 KDa
Source organism: synthetic construct
Expression system: Not provided
DNA (5'-D(*GP*CP*CP*CP*GP*TP*CP*C)-3') Chain: C
Molecule details ›
Chain: C
Length: 8 nucleotides
Theoretical weight: 2.36 KDa
Source organism: synthetic construct
Expression system: Not provided
DNA (5'-D(*AP*CP*TP*TP*TP*GP*AP*GP*GP*CP*AP*GP*AP*G)-3') Chain: D
Molecule details ›
Chain: D
Length: 14 nucleotides
Theoretical weight: 4.34 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand K 1 x K
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U1
Spacegroup: P212121
Unit cell:
a: 47.67Å b: 92.73Å c: 102.78Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.237 0.235 0.269
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

3 review citations

Small-Molecule Inhibitors Targeting FEN1 for Cancer Therapy.
Yang et al. (2022)
2 more

1 mention without citation

Structural basis of 5' flap recognition and protein-protein interactions of human flap endonuclease 1.
Xu et al. (2018)