› 5v95

X-ray diffraction
2.3Å resolution

Structure of the H477R variant of rat cytosolic PEPCK in complex with manganese.

Released:
DOI: 10.2210/pdb5v95/pdb
PDB entry 5v95 coloured by chain, front view.
PDB entry 5v95 coloured by chain, side view.
PDB entry 5v95 coloured by chain, top view.
Source organism: Rattus norvegicus
Primary publication:
Asymmetric Anchoring Is Required for Efficient Ω-Loop Opening and Closing in Cytosolic Phosphoenolpyruvate Carboxykinase.
Cui DS, Broom A, Mcleod MJ, Meiering EM, Holyoak T
Biochemistry 56 2106-2115 (2017)
PMID: 28345895

Function and Biology Details

Reaction catalysed: 
GTP + oxaloacetate = GDP + phosphoenolpyruvate + CO(2)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-139574 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phosphoenolpyruvate carboxykinase, cytosolic [GTP] Chain: A
Molecule details ›
Chain: A
Length: 622 amino acids
Theoretical weight: 69.52 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli
UniProt:
  • Canonical: P07379 (Residues: 1-622; Coverage: 100%)
Gene name: Pck1
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand MN 2 x MN
Ligand NA 1 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: P21
Unit cell:
a: 46.152Å b: 118.199Å c: 61.47Å
α: 90° β: 111.35° γ: 90°
R-values:
R R work R free
0.218 0.215 0.261
Expression system: Escherichia coli

Quick links

Citations

4 citation in other articles

Dynamic Acetylation of Phosphoenolpyruvate Carboxykinase Toggles Enzyme Activity between Gluconeogenic and Anaplerotic Reactions.
Latorre-Muro et al. (2018)
3 more