5uul

X-ray diffraction
1.33Å resolution

Human Bfl-1 in complex with PUMA BH3

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
PDBe Complex ID:
PDB-CPX-172492 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Bcl-2-related protein A1 Chain: A
Molecule details ›
Chain: A
Length: 152 amino acids
Theoretical weight: 17.44 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q16548 (Residues: 1-151; Coverage: 86%)
Gene names: BCL2A1, BCL2L5, BFL1, GRS, HBPA1
Sequence domains: Apoptosis regulator proteins, Bcl-2 family
Structure domains: Blc2-like
Bcl-2-binding component 3, isoforms 1/2 Chain: B
Molecule details ›
Chain: B
Length: 25 amino acids
Theoretical weight: 2.82 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: Q9BXH1 (Residues: 132-154; Coverage: 12%)
Gene names: BBC3, PUMA

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P21
Unit cell:
a: 43.213Å b: 43.428Å c: 47.384Å
α: 90° β: 114.39° γ: 90°
R-values:
R R work R free
0.134 0.132 0.155
Expression systems:
  • Escherichia coli
  • Not provided