› 5usz

X-ray diffraction
2.1Å resolution

JAK2 JH2 in complex with JNJ-7706621

Released:
DOI: 10.2210/pdb5usz/pdb
PDB entry 5usz coloured by chain, front view.
PDB entry 5usz coloured by chain, side view.
PDB entry 5usz coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Identification and Characterization of JAK2 Pseudokinase Domain Small Molecule Binders.
Puleo DE, Kucera K, Hammarén HM, Ungureanu D, Newton AS, Silvennoinen O, Jorgensen WL, Schlessinger J
ACS Med Chem Lett 8 618-621 (2017)
PMID: 28626521

Function and Biology Details

Reaction catalysed: 
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-130063 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Tyrosine-protein kinase JAK2 Chain: A
Molecule details ›
Chain: A
Length: 289 amino acids
Theoretical weight: 33.12 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: O60674 (Residues: 536-812; Coverage: 25%)
Gene name: JAK2
Sequence domains: Protein tyrosine and serine/threonine kinase
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand SKE 1 x SKE
Ligand GOL 2 x GOL
Ligand DMS 1 x DMS
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: P21
Unit cell:
a: 44.716Å b: 57.533Å c: 60.769Å
α: 90° β: 110.41° γ: 90°
R-values:
R R work R free
0.174 0.172 0.224
Expression system: Spodoptera frugiperda

Quick links

Citations

4 review citations

Janus kinases to jakinibs: from basic insights to clinical practice.
Gadina et al. (2019)
3 more

6 mentions without citation

Prospects for pharmacological targeting of pseudokinases.
Kung et al. (2019)
5 more