› 5un7

X-ray diffraction
2.1Å resolution

Structure of the human POT1-TPP1 telomeric complex

Released:
DOI: 10.2210/pdb5un7/pdb
PDB entry 5un7 coloured by chain, front view.
PDB entry 5un7 coloured by chain, side view.
PDB entry 5un7 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural and functional analysis of the human POT1-TPP1 telomeric complex.
Rice C, Shastrula PK, Kossenkov AV, Hills R, Baird DM, Showe LC, Doukov T, Janicki S, Skordalakes E
Nat Commun 8 14928 (2017)
PMID: 28393830

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
hetero dimer (preferred)
hetero tetramer
Assembly name:
PDBe Complex ID:
PDB-CPX-188359 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Protection of telomeres protein 1 Chain: A
Molecule details ›
Chain: A
Length: 308 amino acids
Theoretical weight: 34.79 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9NUX5 (Residues: 330-634; Coverage: 48%)
Gene name: POT1
Sequence domains: Protection of telomeres protein 1 (POT1), C-terminal insertion domain
Adrenocortical dysplasia protein homolog Chain: B
Molecule details ›
Chain: B
Length: 86 amino acids
Theoretical weight: 9.2 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q96AP0 (Residues: 169-251; Coverage: 18%)
Gene names: ACD, PIP1, PTOP, TINT1, TPP1

Ligands and Environments

2 bound ligands:
Ligand ZN 1 x ZN
Ligand K 3 x K
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL12-2
Spacegroup: P4122
Unit cell:
a: 99.53Å b: 99.53Å c: 122.14Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.184 0.182 0.222
Expression system: Escherichia coli

Quick links

Citations

18 review citations

Shelterin-Mediated Telomere Protection.
de Lange T. (2018)
17 more

10 mentions without citation

Structural biology of telomeres and telomerase.
Smith et al. (2020)
9 more