› 5ugl

X-ray diffraction
1.86Å resolution

Crystal Structure of FGF Receptor 2 Tyrosine Kinase Domain Harboring the D650V Activating Mutation

Released:
DOI: 10.2210/pdb5ugl/pdb
PDB entry 5ugl coloured by chain, front view.
PDB entry 5ugl coloured by chain, side view.
PDB entry 5ugl coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Elucidation of a four-site allosteric network in fibroblast growth factor receptor tyrosine kinases.
Chen H, Marsiglia WM, Cho MK, Huang Z, Deng J, Blais SP, Gai W, Bhattacharya S, Neubert TA, Traaseth NJ, Mohammadi M
Elife 6 (2017)
PMID: 28166054

Function and Biology Details

Reaction catalysed: 
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-149426 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Fibroblast growth factor receptor 2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 324 amino acids
Theoretical weight: 37.09 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P21802 (Residues: 458-768; Coverage: 39%)
Gene names: BEK, FGFR2, KGFR, KSAM
Sequence domains: Protein tyrosine and serine/threonine kinase
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand SO4 2 x SO4
Ligand ANP 2 x ANP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4A
Spacegroup: P212121
Unit cell:
a: 67.215Å b: 78.786Å c: 116.544Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.221 0.219 0.258
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Structure, activation and dysregulation of fibroblast growth factor receptor kinases: perspectives for clinical targeting.
Farrell et al. (2018)
3 more

1 mention without citation

Elucidation of a four-site allosteric network in fibroblast growth factor receptor tyrosine kinases.
Chen et al. (2017)