› 5twy

X-ray diffraction
2.91Å resolution

Structure of Maternal Embryonic Leucine Zipper Kinase

Released:
DOI: 10.2210/pdb5twy/pdb
PDB entry 5twy coloured by chain, front view.
PDB entry 5twy coloured by chain, side view.
PDB entry 5twy coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
MELK is not necessary for the proliferation of basal-like breast cancer cells.
Huang HT, Seo HS, Zhang T, Wang Y, Jiang B, Li Q, Buckley DL, Nabet B, Roberts JM, Paulk J, Dastjerdi S, Winter GE, McLauchlan H, Moran J, Bradner JE, Eck MJ, Dhe-Paganon S, Zhao JJ, Gray NS
Elife 6 (2017)
PMID: 28926338

Function and Biology Details

Reactions catalysed: 
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-172082 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Maternal embryonic leucine zipper kinase Chains: A, B
Molecule details ›
Chains: A, B
Length: 341 amino acids
Theoretical weight: 39.38 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q14680 (Residues: 2-340; Coverage: 52%)
Gene names: KIAA0175, MELK
Sequence domains:

Ligands and Environments

1 bound ligand:
Ligand 7LY 2 x 7LY
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P21
Unit cell:
a: 61.86Å b: 80.02Å c: 79.8Å
α: 90° β: 109.95° γ: 90°
R-values:
R R work R free
0.288 0.286 0.33
Expression system: Escherichia coli BL21(DE3)

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Citations

12 review citations

Proteolysis-Targeting Chimeras as Therapeutics and Tools for Biological Discovery.
Burslem et al. (2020)
11 more