› 5tua

X-ray diffraction
3.3Å resolution

structure of a Na+-selective mutant of two-pore channel from Arabidopsis thaliana AtTPC1

Released:
DOI: 10.2210/pdb5tua/pdb
PDB entry 5tua coloured by chain, front view.
PDB entry 5tua coloured by chain, side view.
PDB entry 5tua coloured by chain, top view.
Source organism: Arabidopsis thaliana
Primary publication:
Tuning the ion selectivity of two-pore channels.
Guo J, Zeng W, Jiang Y
Proc Natl Acad Sci U S A 114 1009-1014 (2017)
PMID: 28096396

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-188240 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Two pore calcium channel protein 1 Chain: A
Molecule details ›
Chain: A
Length: 739 amino acids
Theoretical weight: 85.48 KDa
Source organism: Arabidopsis thaliana
Expression system: Komagataella pastoris
UniProt:
  • Canonical: Q94KI8 (Residues: 1-733; Coverage: 100%)
Gene names: At4g03560, CCH1, F9H3.19, FOU2, T5L23.5, TPC1
Sequence domains: Ion transport protein

Ligands and Environments

3 bound ligands:
Ligand CA 2 x CA
Ligand BA 9 x BA
Ligand NA 1 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-D
Spacegroup: C2221
Unit cell:
a: 88.535Å b: 156.371Å c: 217.128Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.311 0.31 0.323
Expression system: Komagataella pastoris

Quick links

Citations

23 review citations

Calcium transport across plant membranes: mechanisms and functions.
Demidchik et al. (2018)
22 more

2 mentions without citation

Tuning the ion selectivity of two-pore channels.
Guo et al. (2017)
1 more