5tjg

X-ray diffraction
2.6Å resolution

Thermus aquaticus delta1.1-sigmaA holoenzyme/downstream-fork promoter complex with an open clamp

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero heptamer (preferred)
PDBe Complex ID:
PDB-CPX-119153 (preferred)
Entry contents:
5 distinct polypeptide molecules
1 distinct DNA molecule
Macromolecules (6 distinct):
DNA-directed RNA polymerase subunit alpha Chains: A, B
Molecule details ›
Chains: A, B
Length: 314 amino acids
Theoretical weight: 34.93 KDa
Source organism: Thermus aquaticus
UniProt:
  • Canonical: Q9KWU8 (Residues: 1-314; Coverage: 100%)
Gene name: rpoA
Sequence domains:
Structure domains:
DNA-directed RNA polymerase subunit beta Chain: C
Molecule details ›
Chain: C
Length: 1119 amino acids
Theoretical weight: 124.93 KDa
Source organism: Thermus aquaticus
UniProt:
  • Canonical: Q9KWU7 (Residues: 1-1119; Coverage: 100%)
Gene name: rpoB
Sequence domains:
DNA-directed RNA polymerase subunit beta' Chain: D
Molecule details ›
Chain: D
Length: 1524 amino acids
Theoretical weight: 171.15 KDa
Source organism: Thermus aquaticus
UniProt:
  • Canonical: Q9KWU6 (Residues: 1-1524; Coverage: 100%)
Gene name: rpoC
Sequence domains:
DNA-directed RNA polymerase subunit omega Chain: E
Molecule details ›
Chain: E
Length: 99 amino acids
Theoretical weight: 11.64 KDa
Source organism: Thermus aquaticus
UniProt:
  • Canonical: Q9EVV4 (Residues: 1-99; Coverage: 100%)
Gene name: rpoZ
Sequence domains: RNA polymerase Rpb6
Structure domains: Eukaryotic RPB6 RNA polymerase subunit
RNA polymerase sigma factor SigA Chain: F
Molecule details ›
Chain: F
Length: 347 amino acids
Theoretical weight: 39.86 KDa
Source organism: Thermus aquaticus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9EZJ8 (Residues: 92-438; Coverage: 79%)
Gene name: sigA
Sequence domains:
DNA (5'-D(*TP*AP*TP*AP*AP*TP*GP*GP*GP*A)-3') Chain: G
Molecule details ›
Chain: G
Length: 31 nucleotides
Theoretical weight: 9.68 KDa

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P1
Unit cell:
a: 104.098Å b: 111.423Å c: 143.177Å
α: 104.12° β: 99.66° γ: 109.43°
R-values:
R R work R free
0.226 0.224 0.262
Expression system: Escherichia coli BL21(DE3)