› 5tf6

X-ray diffraction
2.3Å resolution

Structure and conformational plasticity of the U6 small nuclear ribonucleoprotein core

Released:
DOI: 10.2210/pdb5tf6/pdb
PDB entry 5tf6 coloured by chain, front view.
PDB entry 5tf6 coloured by chain, side view.
PDB entry 5tf6 coloured by chain, top view.
Source organisms:
Primary publication:
Structure and conformational plasticity of the U6 small nuclear ribonucleoprotein core.
Montemayor EJ, Didychuk AL, Liao H, Hu P, Brow DA, Butcher SE
Acta Crystallogr D Struct Biol 73 1-8 (2017)
PMID: 28045380

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-156106 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
U4/U6 snRNA-associated-splicing factor PRP24 Chains: A, C
Molecule details ›
Chains: A, C
Length: 374 amino acids
Theoretical weight: 43.25 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli
UniProt:
  • Canonical: P49960 (Residues: 34-400; Coverage: 83%)
Gene names: PRP24, YM8156.10C, YMR268C
Sequence domains:
Structure domains: Alpha-Beta Plaits
U6 snRNA Chains: B, D
Molecule details ›
Chains: B, D
Length: 72 nucleotides
Theoretical weight: 23.17 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Not provided
Sequence domains: U6 spliceosomal RNA

Ligands and Environments

3 bound ligands:
Ligand K 5 x K
Ligand CL 4 x CL
Ligand GOL 5 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P212121
Unit cell:
a: 76.921Å b: 84.446Å c: 255.142Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.186 0.185 0.232
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

2 review citations

The life of U6 small nuclear RNA, from cradle to grave.
Didychuk et al. (2018)
1 more

3 mentions without citation

Structure and conformational plasticity of the U6 small nuclear ribonucleoprotein core.
Montemayor et al. (2017)
2 more