› 5tao

X-ray diffraction
2.1Å resolution

Haloferax volcanii Malate Synthase Lead(II) complex

Released:
DOI: 10.2210/pdb5tao/pdb
PDB entry 5tao coloured by chain, front view.
PDB entry 5tao coloured by chain, side view.
PDB entry 5tao coloured by chain, top view.
Source organism: Haloferax volcanii DS2
Primary publication:
X-ray analysis of Lead(II) binding to Haloferax volcanii Malate Synthase
Adams MJ, Howard BR
The Journal of the Utah Academy 93 77-96 (2016)

Function and Biology Details

Reaction catalysed: 
Acetyl-CoA + H(2)O + glyoxylate = (S)-malate + CoA
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
homo hexamer
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-112622 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Malate synthase Chain: A
Molecule details ›
Chain: A
Length: 433 amino acids
Theoretical weight: 48.07 KDa
Source organism: Haloferax volcanii DS2
Expression system: Haloferax volcanii
UniProt:
  • Canonical: D4GTL2 (Residues: 1-433; Coverage: 100%)
Gene names: C498_05196, HVO_1983, aceB, aceB1
Structure domains:

Ligands and Environments

6 bound ligands:
Ligand ACT 1 x ACT
Ligand GLV 3 x GLV
Ligand PB 4 x PB
Ligand K 3 x K
Ligand CL 4 x CL
Ligand NA 1 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: R32
Unit cell:
a: 155.353Å b: 155.353Å c: 139.412Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.185 0.183 0.217
Expression system: Haloferax volcanii

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