› 5szg

X-ray diffraction
2.7Å resolution

Structure of the bMERB domain of Mical-3

Released:
DOI: 10.2210/pdb5szg/pdb
PDB entry 5szg coloured by chain, front view.
PDB entry 5szg coloured by chain, side view.
PDB entry 5szg coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
bMERB domains are bivalent Rab8 family effectors evolved by gene duplication.
Rai A, Oprisko A, Campos J, Fu Y, Friese T, Itzen A, Goody RS, Gazdag EM, Müller MP
Elife 5 (2016)
PMID: 27552051

Function and Biology Details

Reaction catalysed: 
[F-actin]-L-methionine + NADPH + O(2) + H(+) = [F-actin]-L-methionine-(R)-S-oxide + NADP(+) + H(2)O
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-181907 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
[F-actin]-monooxygenase MICAL3 Chains: A, B
Molecule details ›
Chains: A, B
Length: 151 amino acids
Theoretical weight: 18.54 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q7RTP6 (Residues: 1841-1990; Coverage: 8%)
Gene names: KIAA0819, KIAA1364, MICAL3
Sequence domains: Bivalent Mical/EHBP Rab binding domain

Ligands and Environments

1 bound ligand:
Ligand PEG 2 x PEG
1 modified residue:
Ligand MSE 12 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: P212121
Unit cell:
a: 51.858Å b: 78.848Å c: 95.557Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.252 0.251 0.282
Expression system: Escherichia coli

Quick links

Citations

5 review citations

Many roads to symmetry breaking: molecular mechanisms and theoretical models of yeast cell polarity.
Goryachev et al. (2017)
4 more

4 mentions without citation

bMERB domains are bivalent Rab8 family effectors evolved by gene duplication.
Rai et al. (2016)
3 more