› 5nj8

X-ray diffraction
3.3Å resolution

Structural basis for aryl hydrocarbon receptor mediated gene activation

Released:
DOI: 10.2210/pdb5nj8/pdb
PDB entry 5nj8 coloured by chain, front view.
PDB entry 5nj8 coloured by chain, side view.
PDB entry 5nj8 coloured by chain, top view.
Source organisms:
Primary publication:
Structural Basis for Aryl Hydrocarbon Receptor-Mediated Gene Activation.
Schulte KW, Green E, Wilz A, Platten M, Daumke O
Structure 25 1025-1033.e3 (2017)
PMID: 28602820

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-119060 (preferred)
Entry contents:
2 distinct polypeptide molecules
2 distinct DNA molecules
Macromolecules (4 distinct):
Aryl hydrocarbon receptor Chains: A, C
Molecule details ›
Chains: A, C
Length: 254 amino acids
Theoretical weight: 28.42 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P35869 (Residues: 23-273; Coverage: 30%)
Gene names: AHR, BHLHE76
Sequence domains:
Aryl hydrocarbon receptor nuclear translocator Chains: B, D
Molecule details ›
Chains: B, D
Length: 239 amino acids
Theoretical weight: 26.47 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: P27540 (Residues: 85-345; Coverage: 30%)
Gene names: ARNT, BHLHE2
Sequence domains:
DNA (5'-D(*GP*GP*TP*CP*AP*CP*GP*CP*AP*AP*CP*C)-3') Chains: E, G
Molecule details ›
Chains: E, G
Length: 12 nucleotides
Theoretical weight: 3.63 KDa
DNA (5'-D(*GP*GP*TP*TP*GP*CP*GP*TP*GP*AP*CP*C)-3') Chains: F, H
Molecule details ›
Chains: F, H
Length: 12 nucleotides
Theoretical weight: 3.69 KDa

Ligands and Environments

2 bound ligands:
Ligand ER3 12 x ER3
Ligand ACT 1 x ACT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.1
Spacegroup: P6522
Unit cell:
a: 91.321Å b: 91.321Å c: 464.914Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.294 0.292 0.333
Expression systems:
  • Escherichia coli BL21(DE3)
  • Escherichia coli

Quick links

Citations

13 review citations

Nutritional Therapy to Modulate Tryptophan Metabolism and Aryl Hydrocarbon-Receptor Signaling Activation in Human Diseases.
Ghiboub et al. (2020)
12 more

16 mentions without citation

bHLH-PAS Proteins: Their Structure and Intrinsic Disorder.
Kolonko et al. (2019)
15 more