› 5myo

X-ray diffraction
1.59Å resolution

Structure of Pyroglutamate-Abeta-specific Fab c#6 in complex with human Abeta-pE3-12-PEGb

Released:
DOI: 10.2210/pdb5myo/pdb
PDB entry 5myo coloured by chain, front view.
PDB entry 5myo coloured by chain, side view.
PDB entry 5myo coloured by chain, top view.
Source organisms:
Primary publication:
Structural and functional analyses of pyroglutamate-amyloid-β-specific antibodies as a basis for Alzheimer immunotherapy.
Piechotta A, Parthier C, Kleinschmidt M, Gnoth K, Pillot T, Lues I, Demuth HU, Schilling S, Rahfeld JU, Stubbs MT
J Biol Chem 292 12713-12724 (2017)
PMID: 28623233

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
hetero trimer (preferred)
hetero dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-209168 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Fab c#6 light chain Chains: A, C
Molecule details ›
Chains: A, C
Length: 219 amino acids
Theoretical weight: 24.17 KDa
Source organism: Mus musculus
Expression system: Mus musculus
Structure domains: Immunoglobulins
Fab c#6 heavy chain Chains: B, D
Molecule details ›
Chains: B, D
Length: 228 amino acids
Theoretical weight: 24.68 KDa
Source organism: Mus musculus
Expression system: Mus musculus
Structure domains: Immunoglobulins
Amyloid-beta protein 40 Chain: E
Molecule details ›
Chain: E
Length: 10 amino acids
Theoretical weight: 1.22 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P05067 (Residues: 674-683; Coverage: 1%)
Gene names: A4, AD1, APP

Ligands and Environments

2 bound ligands:
Ligand SO4 1 x SO4
Ligand GOL 3 x GOL
1 modified residue:
Ligand PCA 1 x PCA

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007
Spacegroup: P1
Unit cell:
a: 53.774Å b: 65.958Å c: 67.76Å
α: 62.87° β: 82.93° γ: 84.11°
R-values:
R R work R free
0.18 0.178 0.215
Expression systems:
  • Mus musculus
  • Not provided

Quick links

Citations

2 review citations

The Amyloid-β Oligomer Hypothesis: Beginning of the Third Decade.
Cline et al. (2018)
1 more

1 mention without citation

Structural and functional analyses of pyroglutamate-amyloid-β-specific antibodies as a basis for Alzheimer immunotherapy.
Piechotta et al. (2017)