› 5ms3

X-ray diffraction
2.3Å resolution

Kallikrein-related peptidase 8 calcium complex

Released:
DOI: 10.2210/pdb5ms3/pdb
PDB entry 5ms3 coloured by chain, front view.
PDB entry 5ms3 coloured by chain, side view.
PDB entry 5ms3 coloured by chain, top view.
Source organism: Homo sapiens
Entry authors: Debela M, Magdolen V, Skala W, Bode W, Brandstetter H, Goettig P

Function and Biology Details

Reaction catalysed: 
Cleavage of amide substrates following the basic amino acids Arg or Lys at the P1 position, with a preference for Arg over Lys
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-129924 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Kallikrein-8 Chain: A
Molecule details ›
Chain: A
Length: 228 amino acids
Theoretical weight: 24.48 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: O60259 (Residues: 33-260; Coverage: 98%)
Gene names: KLK8, NRPN, PRSS19, TADG14, UNQ283/PRO322
Sequence domains: Trypsin
Structure domains: Trypsin-like serine proteases

Ligands and Environments

1 bound ligand:
Ligand CA 1 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE X12
Spacegroup: P212121
Unit cell:
a: 45.997Å b: 51.741Å c: 82.863Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.223 0.221 0.266
Expression system: Escherichia coli

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