› 5m6b

X-ray diffraction
3.25Å resolution

structure of recombinant mushroom tyrosinase (abPPO4)

Released:
DOI: 10.2210/pdb5m6b/pdb
PDB entry 5m6b coloured by chain, front view.
PDB entry 5m6b coloured by chain, side view.
PDB entry 5m6b coloured by chain, top view.
Source organism: Agaricus bisporus
Primary publication:
Heterologous expression and characterization of functional mushroom tyrosinase (AbPPO4).
Pretzler M, Bijelic A, Rompel A
Sci Rep 7 1810 (2017)
PMID: 28500345

Function and Biology Details

Reaction catalysed: 
(1a) L-tyrosine + 1/2 O(2) = L-dopa
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-111701 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Polyphenol oxidase 4 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 574 amino acids
Theoretical weight: 65.18 KDa
Source organism: Agaricus bisporus
Expression system: Escherichia coli
UniProt:
  • Canonical: C7FF05 (Residues: 1-565; Coverage: 93%)
Gene name: PPO4
Sequence domains:
Structure domains: Di-copper center containing domain from catechol oxidase

Ligands and Environments

2 bound ligands:
Ligand CU 8 x CU
Ligand O 1 x O
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: C2
Unit cell:
a: 287.3Å b: 52.09Å c: 152.66Å
α: 90° β: 98.03° γ: 90°
R-values:
R R work R free
0.252 0.251 0.275
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Ten Good Reasons for the Use of the Tellurium-Centered Anderson-Evans Polyoxotungstate in Protein Crystallography.
Bijelic et al. (2017)
2 more

8 mentions without citation

Heterologous expression and characterization of functional mushroom tyrosinase (AbPPO4).
Pretzler et al. (2017)
7 more