› 5lhf

X-ray diffraction
1.75Å resolution

Phosphoribosyl anthranilate isomerase from Thermococcus kodakaraensis

Released:
DOI: 10.2210/pdb5lhf/pdb
PDB entry 5lhf coloured by chain, front view.
PDB entry 5lhf coloured by chain, side view.
PDB entry 5lhf coloured by chain, top view.
Source organism: Thermococcus kodakarensis KOD1
Primary publication:
Crystal structure of a phosphoribosyl anthranilate isomerase from the hyperthermophilic archaeon Thermococcus kodakaraensis.
Perveen S, Rashid N, Papageorgiou AC
Acta Crystallogr F Struct Biol Commun 72 804-812 (2016)
PMID: 27827353

Function and Biology Details

Reaction catalysed: 
N-(5-phospho-beta-D-ribosyl)anthranilate = 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-195501 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
N-(5'-phosphoribosyl)anthranilate isomerase Chains: A, B
Molecule details ›
Chains: A, B
Length: 209 amino acids
Theoretical weight: 23.22 KDa
Source organism: Thermococcus kodakarensis KOD1
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9YGB1 (Residues: 1-208; Coverage: 100%)
Gene names: TK0256, trpF
Sequence domains: N-(5'phosphoribosyl)anthranilate (PRA) isomerase
Structure domains: Aldolase class I

Ligands and Environments

2 bound ligands:
Ligand CL 2 x CL
Ligand NA 2 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE MASSIF-1
Spacegroup: P1
Unit cell:
a: 29.678Å b: 47.144Å c: 88.518Å
α: 102.61° β: 93.88° γ: 108.34°
R-values:
R R work R free
0.175 0.173 0.214
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 review citation

An overview of 25 years of research on Thermococcus kodakarensis, a genetically versatile model organism for archaeal research.
Rashid et al. (2020)
1 other article

1 mention without citation

Crystal structure of a phosphoribosyl anthranilate isomerase from the hyperthermophilic archaeon Thermococcus kodakaraensis.
Perveen et al. (2016)