› 5lbx

X-ray diffraction
2.5Å resolution

Structure of the T175V Etr1p mutant in the trigonal form P312 in complex with NADP and crotonyl-CoA

Released:
DOI: 10.2210/pdb5lbx/pdb
PDB entry 5lbx coloured by chain, front view.
PDB entry 5lbx coloured by chain, side view.
PDB entry 5lbx coloured by chain, top view.
Source organism: Candida tropicalis
Primary publication:
A conserved threonine prevents self-intoxication of enoyl-thioester reductases.
Rosenthal RG, Vögeli B, Wagner T, Shima S, Erb TJ
Nat Chem Biol 13 745-749 (2017)
PMID: 28504678

Function and Biology Details

Reaction catalysed: 
An acyl-[acyl-carrier protein] + NADP(+) = a trans-2,3-dehydroacyl-[acyl-carrier protein] + NADPH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-186819 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Enoyl-[acyl-carrier-protein] reductase 1, mitochondrial Chains: A, B
Molecule details ›
Chains: A, B
Length: 385 amino acids
Theoretical weight: 42.09 KDa
Source organism: Candida tropicalis
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: Q8WZM3 (Residues: 23-386; Coverage: 94%)
Gene name: ETR1
Sequence domains: Zinc-binding dehydrogenase
Structure domains:

Ligands and Environments

4 bound ligands:
Ligand NAP 2 x NAP
Ligand SO4 13 x SO4
Ligand COO 2 x COO
Ligand CL 1 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: P3121
Unit cell:
a: 93.498Å b: 93.498Å c: 231.203Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.184 0.182 0.212
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'

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Citations

2 review citations

A short history of RubisCO: the rise and fall (?) of Nature's predominant CO2 fixing enzyme.
Erb et al. (2018)
1 more