› 5kzf

X-ray diffraction
3.49Å resolution

Crystal structure of near full-length hexameric Mycobacterium tuberculosis proteasomal ATPase Mpa in apo form

Released:
DOI: 10.2210/pdb5kzf/pdb
PDB entry 5kzf coloured by chain, front view.
PDB entry 5kzf coloured by chain, side view.
PDB entry 5kzf coloured by chain, top view.
Source organism: Mycobacterium tuberculosis H37Ra
Primary publication:
Mycobacterium tuberculosis proteasomal ATPase Mpa has a β-grasp domain that hinders docking with the proteasome core protease.
Wu Y, Hu K, Li D, Bai L, Yang S, Jastrab JB, Xiao S, Hu Y, Zhang S, Darwin KH, Wang T, Li H
Mol Microbiol 105 227-241 (2017)
PMID: 28419599

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-107737 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Proteasome-associated ATPase Chains: A, B, C, D, E, F, G, H, I, J, K, L
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J, K, L
Length: 513 amino acids
Theoretical weight: 56.85 KDa
Source organism: Mycobacterium tuberculosis H37Ra
Expression system: Escherichia coli
UniProt:
  • Canonical: A5U4E1 (Residues: 98-609; Coverage: 84%)
Gene names: MRA_2130, mpa
Sequence domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25
Spacegroup: P212121
Unit cell:
a: 115.265Å b: 202.59Å c: 303.007Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.269 0.268 0.306
Expression system: Escherichia coli

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Citations

5 review citations

Targeting the Proteostasis Network for Mycobacterial Drug Discovery.
Lupoli et al. (2018)
4 more

3 mentions without citation

Biology and Biochemistry of Bacterial Proteasomes.
Becker et al. (2019)
2 more