› 5ksy

X-ray diffraction
2.48Å resolution

hMiro1 C-domain GDP Complex P41212 Crystal Form

Released:
DOI: 10.2210/pdb5ksy/pdb
PDB entry 5ksy coloured by chain, front view.
PDB entry 5ksy coloured by chain, side view.
PDB entry 5ksy coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural insights into Parkin substrate lysine targeting from minimal Miro substrates.
Klosowiak JL, Park S, Smith KP, French ME, Focia PJ, Freymann DM, Rice SE
Sci Rep 6 33019 (2016)
PMID: 27605430

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-184913 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Mitochondrial Rho GTPase 1 Chain: A
Molecule details ›
Chain: A
Length: 192 amino acids
Theoretical weight: 22.19 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8IXI2 (Residues: 411-592; Coverage: 29%)
Gene names: ARHT1, RHOT1
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

1 bound ligand:
Ligand GDP 1 x GDP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-D
Spacegroup: P41212
Unit cell:
a: 51.9Å b: 51.9Å c: 151.06Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.271 0.266 0.315
Expression system: Escherichia coli

Quick links

Citations

10 review citations

RBR ligase-mediated ubiquitin transfer: a tale with many twists and turns.
Walden et al. (2018)
9 more

1 mention without citation

Structural insights into Parkin substrate lysine targeting from minimal Miro substrates.
Klosowiak et al. (2016)