› 5khk

X-ray diffraction
2.07Å resolution

HCN2 CNBD in complex with 2-aminopurine riboside-3', 5'-cyclic monophosphate (2-NH2-cPuMP)

Released:
DOI: 10.2210/pdb5khk/pdb
PDB entry 5khk coloured by chain, front view.
PDB entry 5khk coloured by chain, side view.
PDB entry 5khk coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Cyclic Purine and Pyrimidine Nucleotides Bind to the HCN2 Ion Channel and Variably Promote C-Terminal Domain Interactions and Opening.
Ng LCT, Putrenko I, Baronas V, Van Petegem F, Accili EA
Structure 24 1629-1642 (2016)
PMID: 27568927

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-131576 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 2 Chain: A
Molecule details ›
Chain: A
Length: 204 amino acids
Theoretical weight: 23.88 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O88703 (Residues: 443-643; Coverage: 23%)
Gene names: Bcng2, Hac1, Hcn2
Sequence domains: Cyclic nucleotide-binding domain
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand 6SZ 1 x 6SZ
1 modified residue:
Ligand CSX 1 x CSX

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-D
Spacegroup: P4212
Unit cell:
a: 96.429Å b: 96.429Å c: 46.423Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.218 0.216 0.261
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 review citations

The Hyperpolarization-Activated Cyclic Nucleotide-Gated Channels: from Biophysics to Pharmacology of a Unique Family of Ion Channels.
Sartiani et al. (2017)
2 more