› 5k33

X-ray diffraction
3.3Å resolution

Crystal structure of extracellular domain of HER2 in complex with Fcab STAB19

Released:
DOI: 10.2210/pdb5k33/pdb
PDB entry 5k33 coloured by chain, front view.
PDB entry 5k33 coloured by chain, side view.
PDB entry 5k33 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Fcab-HER2 Interaction: a Ménage à Trois. Lessons from X-Ray and Solution Studies.
Lobner E, Humm AS, Göritzer K, Mlynek G, Puchinger MG, Hasenhindl C, Rüker F, Traxlmayr MW, Djinović-Carugo K, Obinger C
Structure 25 878-889.e5 (2017)
PMID: 28528777

Function and Biology Details

Reaction catalysed: 
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-134853 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (4 distinct):
Immunoglobulin heavy constant gamma 1 Chain: A
Molecule details ›
Chain: A
Length: 227 amino acids
Theoretical weight: 25.57 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P01857 (Residues: 108-295, 296-328; Coverage: 55%)
Gene name: IGHG1
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins
Receptor tyrosine-protein kinase erbB-2 Chain: C
Molecule details ›
Chain: C
Length: 607 amino acids
Theoretical weight: 66.99 KDa
Source organism: Homo sapiens
Expression system: Cricetulus griseus
UniProt:
  • Canonical: P04626 (Residues: 23-629; Coverage: 49%)
Gene names: ERBB2, HER2, MLN19, NEU, NGL
Sequence domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer
2 bound ligands:
Ligand GOL 1 x GOL
Ligand NAG 2 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE MASSIF-3
Spacegroup: P3221
Unit cell:
a: 108.62Å b: 108.62Å c: 174.34Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.222 0.218 0.271
Expression systems:
  • Homo sapiens
  • Cricetulus griseus

Quick links

Citations

2 review citations

Label-free methods for optical in vitro characterization of protein-protein interactions.
Soltermann et al. (2021)
1 more

5 mentions without citation

Reliable identification at the species level of Brucella isolates with MALDI-TOF-MS.
Lista et al. (2011)
4 more