› 5k20

X-ray diffraction
2.2Å resolution

Caspase-7 S239E Phosphomimetic

Released:
DOI: 10.2210/pdb5k20/pdb
PDB entry 5k20 coloured by chain, front view.
PDB entry 5k20 coloured by chain, side view.
PDB entry 5k20 coloured by chain, top view.
Source organism: Homo sapiens
Entry authors: Eron SJ, Hardy JA

Function and Biology Details

Reaction catalysed: 
Strict requirement for an Asp residue at position P1 and has a preferred cleavage sequence of Asp-Glu-Val-Asp-|-
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-157234 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Caspase-7 subunit p20 Chains: A, C
Molecule details ›
Chains: A, C
Length: 198 amino acids
Theoretical weight: 22.19 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P55210 (Residues: 1-198; Coverage: 65%)
Gene names: CASP7, MCH3
Sequence domains: Caspase domain
Structure domains: Rossmann fold
Caspase-7 subunit p11 Chains: B, D
Molecule details ›
Chains: B, D
Length: 113 amino acids
Theoretical weight: 13.27 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P55210 (Residues: 199-303; Coverage: 35%)
Gene names: CASP7, MCH3
Sequence domains: Caspase domain
Structure domains: Caspase-like

Ligands and Environments

1 bound ligand:
Ligand FMT 3 x FMT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X6A
Spacegroup: P3221
Unit cell:
a: 88.757Å b: 88.757Å c: 185.141Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.195 0.191 0.228
Expression system: Escherichia coli BL21(DE3)

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