› 5k08

X-ray diffraction
1.4Å resolution

RecA mini intein-Zeise's salt complex

Released:
DOI: 10.2210/pdb5k08/pdb
PDB entry 5k08 coloured by chain, front view.
PDB entry 5k08 coloured by chain, side view.
PDB entry 5k08 coloured by chain, top view.
Source organism: Mycobacterium tuberculosis
Entry authors: Li Z, Li HM

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-161555 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Endonuclease PI-MtuI Chain: A
Molecule details ›
Chain: A
Length: 142 amino acids
Theoretical weight: 15.67 KDa
Source organism: Mycobacterium tuberculosis
Expression system: Escherichia coli
UniProt:
  • Canonical: P9WHJ3 (Residues: 252-345; Coverage: 12%)
Gene names: MTV002.02c, Rv2737c, recA
Structure domains: Hedgehog/Intein (Hint) domain

Ligands and Environments

3 bound ligands:
Ligand ZPT 2 x ZPT
Ligand SO4 1 x SO4
Ligand TCE 1 x TCE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL14-1
Spacegroup: P21212
Unit cell:
a: 57.687Å b: 63.93Å c: 37.307Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.182 0.18 0.21
Expression system: Escherichia coli

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