5jvr

X-ray diffraction
2.1Å resolution

The neck-linker of Mus musculus KIF3A fused to the alpha 7 helix of Drosophila melanogaster Kinesin-1 fused to EB1

Released:

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
PDBe Complex ID:
PDB-CPX-147894 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Kinesin heavy chain; Kinesin-like protein KIF3A; Microtubule-associated protein RP/EB family member 1 Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 75 amino acids
Theoretical weight: 8.99 KDa
Source organisms: Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P28741 (Residues: 352-359; Coverage: 1%)
  • Canonical: Q15691 (Residues: 193-193, 207-257; Coverage: 19%)
  • Canonical: P17210 (Residues: 346-358; Coverage: 1%)
Gene names: CG7765, Khc, Kif3, Kif3a, MAPRE1, kin
Sequence domains: EB1-like C-terminal motif
Structure domains: Single alpha-helices involved in coiled-coils or other helix-helix interfaces

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P212121
Unit cell:
a: 53.172Å b: 80.189Å c: 155.375Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.198 0.196 0.234
Expression system: Escherichia coli BL21(DE3)