› 5jhv

X-ray diffraction
2.75Å resolution

Apo form of influenza strain H1N1 polymerase acidic subunit N-terminal region crystallized with polyethylene glycol 8000

Released:
DOI: 10.2210/pdb5jhv/pdb
PDB entry 5jhv coloured by chain, front view.
PDB entry 5jhv coloured by chain, side view.
PDB entry 5jhv coloured by chain, top view.
Source organism: Influenza A virus (A/Puerto Rico/8/1934(H1N1))
Primary publication:
Influence of Precipitants on Molecular Arrangements and Space Groups of Protein Crystals
Fudo S, Fei Q, Nukaga M, Hoshino T
Cryst Growth Des 17 534-542 (2017)

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-137016 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Polymerase acidic protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 191 amino acids
Theoretical weight: 22.3 KDa
Source organism: Influenza A virus (A/Puerto Rico/8/1934(H1N1))
Expression system: Escherichia coli
UniProt:
  • Canonical: P03433 (Residues: 1-50, 73-197; Coverage: 24%)
Gene name: PA
Structure domains: Influenza RNA-dependent RNA polymerase subunit PA, endonuclease domain

Ligands and Environments

1 bound ligand:
Ligand MN 4 x MN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-5A
Spacegroup: C2
Unit cell:
a: 64.243Å b: 87.404Å c: 66.497Å
α: 90° β: 94.31° γ: 90°
R-values:
R R work R free
0.199 0.197 0.234
Expression system: Escherichia coli

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