› 5jdo

X-ray diffraction
3.2Å resolution

T. congolense haptoglobin-haemoglobin receptor in complex with haemoglobin

Released:
DOI: 10.2210/pdb5jdo/pdb
PDB entry 5jdo coloured by chain, front view.
PDB entry 5jdo coloured by chain, side view.
PDB entry 5jdo coloured by chain, top view.
Source organisms:
Primary publication:
Evolutionary diversification of the trypanosome haptoglobin-haemoglobin receptor from an ancestral haemoglobin receptor.
Lane-Serff H, MacGregor P, Peacock L, Macleod OJ, Kay C, Gibson W, Higgins MK, Carrington M
Elife 5 (2016)
PMID: 27083048

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
PDBe Complex ID:
PDB-CPX-124131 (preferred)
Entry contents:
6 distinct polypeptide molecules
Macromolecules (6 distinct):
Haptoglobin-haemoglobin receptor Chain: A
Molecule details ›
Chain: A
Length: 249 amino acids
Theoretical weight: 26.56 KDa
Source organism: Trypanosoma congolense
Expression system: Escherichia coli
UniProt:
  • Canonical: G0UVW6 (Residues: 116-364; Coverage: 64%)
Gene name: TCIL3000_10_2930
Sequence domains: Haptoglobin-hemoglobin receptor
Structure domains: Ferritin
Haptoglobin-haemoglobin receptor Chain: B
Molecule details ›
Chain: B
Length: 245 amino acids
Theoretical weight: 26.12 KDa
Source organism: Trypanosoma congolense
Expression system: Escherichia coli
UniProt:
  • Canonical: G0UVW6 (Residues: 116-360; Coverage: 63%)
Gene name: TCIL3000_10_2930
Sequence domains: Haptoglobin-hemoglobin receptor
Structure domains: Ferritin
Hemoglobin subunit alpha Chain: C
Molecule details ›
Chain: C
Length: 141 amino acids
Theoretical weight: 15.15 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P69905 (Residues: 2-142; Coverage: 99%)
Gene names: HBA1, HBA2
Sequence domains: Globin
Structure domains: Globins
Hemoglobin subunit beta Chain: D
Molecule details ›
Chain: D
Length: 145 amino acids
Theoretical weight: 15.79 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P68871 (Residues: 3-147; Coverage: 99%)
Gene name: HBB
Sequence domains: Globin
Structure domains: Globins
Hemoglobin subunit alpha Chain: E
Molecule details ›
Chain: E
Length: 140 amino acids
Theoretical weight: 14.99 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P69905 (Residues: 2-141; Coverage: 99%)
Gene names: HBA1, HBA2
Sequence domains: Globin
Structure domains: Globins
Hemoglobin subunit beta Chain: F
Molecule details ›
Chain: F
Length: 144 amino acids
Theoretical weight: 15.65 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P68871 (Residues: 3-146; Coverage: 98%)
Gene name: HBB
Sequence domains: Globin
Structure domains: Globins

Ligands and Environments

2 bound ligands:
Ligand HEM 4 x HEM
Ligand OXY 4 x OXY
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Unit cell:
a: 72.75Å b: 127.3Å c: 172.42Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.206 0.204 0.235
Expression systems:
  • Escherichia coli
  • Homo sapiens

Quick links

Citations

2 review citations

A Receptor's Tale: An Eon in the Life of a Trypanosome Receptor.
Higgins et al. (2017)
1 more

1 mention without citation

Oxygen dissociation from ferrous oxygenated human hemoglobin:haptoglobin complexes confirms that in the R-state α and β chains are functionally heterogeneous.
Ascenzi et al. (2019)