› 5jbn

X-ray diffraction
1.45Å resolution

Crystal Structure of Apo Phosphopantetheine Adenylyltransferase (PPAT/CoaD) from E. coli

Released:
DOI: 10.2210/pdb5jbn/pdb
PDB entry 5jbn coloured by chain, front view.
PDB entry 5jbn coloured by chain, side view.
PDB entry 5jbn coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
Facilitating unambiguous NMR assignments and enabling higher probe density through selective labeling of all methyl containing amino acids.
Proudfoot A, Frank AO, Ruggiu F, Mamo M, Lingel A
J Biomol NMR 65 15-27 (2016)
PMID: 27130242

Function and Biology Details

Reaction catalysed: 
ATP + pantetheine 4'-phosphate = diphosphate + 3'-dephospho-CoA
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-141362 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phosphopantetheine adenylyltransferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 170 amino acids
Theoretical weight: 19.25 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A6I6 (Residues: 1-159; Coverage: 100%)
Gene names: JW3609, b3634, coaD, kdtB, yicA
Sequence domains: Cytidylyltransferase-like
Structure domains: HUPs

Ligands and Environments

2 bound ligands:
Ligand SO4 6 x SO4
Ligand DMS 1 x DMS
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL12-2
Spacegroup: I23
Unit cell:
a: 136.176Å b: 136.176Å c: 136.176Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.147 0.145 0.182
Expression system: Escherichia coli

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Citations

10 citation in other articles

Automatic structure-based NMR methyl resonance assignment in large proteins.
PritiĊĦanac et al. (2019)
9 more