› 5j6g

X-ray diffraction
3.3Å resolution

Recognition of the MHC class Ib molecule H2-Q10 by the natural killer cell receptor Ly49C

Released:
DOI: 10.2210/pdb5j6g/pdb
PDB entry 5j6g coloured by chain, front view.
PDB entry 5j6g coloured by chain, side view.
PDB entry 5j6g coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Recognition of the Major Histocompatibility Complex (MHC) Class Ib Molecule H2-Q10 by the Natural Killer Cell Receptor Ly49C.
Sullivan LC, Berry R, Sosnin N, Widjaja JM, Deuss FA, Balaji GR, LaGruta NL, Mirams M, Trapani JA, Rossjohn J, Brooks AG, Andrews DM
J Biol Chem 291 18740-52 (2016)
PMID: 27385590

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
hetero tetramer (preferred)
hetero octamer
PDBe Complex ID:
PDB-CPX-134982 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
H-2 class I histocompatibility antigen, Q10 alpha chain Chains: A, C
Molecule details ›
Chains: A, C
Length: 300 amino acids
Theoretical weight: 34.59 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: P01898 (Residues: 25-280; Coverage: 85%)
Gene name: H2-Q10
Sequence domains:
Structure domains:
Beta-2-microglobulin Chains: B, D
Molecule details ›
Chains: B, D
Length: 100 amino acids
Theoretical weight: 11.79 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: P01887 (Residues: 21-119; Coverage: 100%)
Gene name: B2m
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins
Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 2 Chains: E, F
Molecule details ›
Chains: E, F
Length: 8 amino acids
Theoretical weight: 830 Da
Source organism: Mus musculus
Expression system: Not provided
UniProt:
  • Canonical: Q9DBG6 (Residues: 373-380; Coverage: 1%)
Gene name: Rpn2
Killer cell lectin-like receptor 3 Chains: G, H
Molecule details ›
Chains: G, H
Length: 132 amino acids
Theoretical weight: 15.78 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q64329 (Residues: 136-266; Coverage: 49%)
Gene names: Klra3, Ly-49c, Ly49C
Sequence domains: Lectin C-type domain
Structure domains: Mannose-Binding Protein A, subunit A

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Unit cell:
a: 101.01Å b: 57.8Å c: 210.704Å
α: 90° β: 96.15° γ: 90°
R-values:
R R work R free
0.222 0.221 0.253
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

2 review citations

Natural Killer Cells: Tumor Surveillance and Signaling.
Meza Guzman et al. (2020)
1 more

2 mentions without citation

Unconventional Peptide Presentation by Classical MHC Class I and Implications for T and NK Cell Activation.
Zajonc DM. (2020)
1 more