› 5j48

X-ray diffraction
1.49Å resolution

PKG I's Carboyl Terminal Cyclic Nucleotide Binding Domain (CNB-B) in a complex with 8-pCPT-cGMP

Released:
DOI: 10.2210/pdb5j48/pdb
PDB entry 5j48 coloured by chain, front view.
PDB entry 5j48 coloured by chain, side view.
PDB entry 5j48 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural Basis of Analog Specificity in PKG I and II.
Campbell JC, Henning P, Franz E, Sankaran B, Herberg FW, Kim C
ACS Chem Biol 12 2388-2398 (2017)
PMID: 28793191

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-171844 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
cGMP-dependent protein kinase 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 135 amino acids
Theoretical weight: 14.99 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q13976 (Residues: 204-336; Coverage: 20%)
Gene names: PRKG1, PRKG1B, PRKGR1A, PRKGR1B
Sequence domains: Cyclic nucleotide-binding domain
Structure domains: Jelly Rolls

Ligands and Environments

4 bound ligands:
Ligand EDO 8 x EDO
Ligand 6FW 2 x 6FW
Ligand NA 2 x NA
Ligand CA 1 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.1
Spacegroup: P21
Unit cell:
a: 35.51Å b: 59.24Å c: 67.32Å
α: 90° β: 100.17° γ: 90°
R-values:
R R work R free
0.165 0.163 0.189
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Cyclic nucleotide selectivity of protein kinase G isozymes.
Kim et al. (2021)
6 other articles

1 mention without citation

Structural Basis of Analog Specificity in PKG I and II.
Campbell et al. (2017)