› 5j11

X-ray diffraction
2.56Å resolution

Structure of human TSLP in complex with TSLPR and IL-7Ralpha

Released:
DOI: 10.2210/pdb5j11/pdb
PDB entry 5j11 coloured by chain, front view.
PDB entry 5j11 coloured by chain, side view.
PDB entry 5j11 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure and antagonism of the receptor complex mediated by human TSLP in allergy and asthma.
Verstraete K, Peelman F, Braun H, Lopez J, Van Rompaey D, Dansercoer A, Vandenberghe I, Pauwels K, Tavernier J, Lambrecht BN, Hammad H, De Winter H, Beyaert R, Lippens G, Savvides SN
Nat Commun 8 14937 (2017)
PMID: 28368013

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-147825 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Thymic stromal lymphopoietin Chain: A
Molecule details ›
Chain: A
Length: 147 amino acids
Theoretical weight: 16.52 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q969D9 (Residues: 8-8, 29-159; Coverage: 81%)
Gene name: TSLP
Sequence domains: Thymic stromal lymphopoietin
Structure domains: Thymic stromal lymphopoietin
Interleukin-7 receptor subunit alpha Chain: B
Molecule details ›
Chain: B
Length: 240 amino acids
Theoretical weight: 27.59 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P16871 (Residues: 21-236; Coverage: 49%)
Gene name: IL7R
Sequence domains:
Structure domains:
Cytokine receptor-like factor 2 Chain: C
Molecule details ›
Chain: C
Length: 230 amino acids
Theoretical weight: 26.57 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q9HC73 (Residues: 1-221; Coverage: 57%)
Gene names: CRL2, CRLF2, ILXR, TSLPR
Sequence domains:
Structure domains: Immunoglobulins

Ligands and Environments

2 bound ligands:
Ligand PGE 1 x PGE
Ligand NAG 2 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SOLEIL BEAMLINE PROXIMA 2
Spacegroup: C2
Unit cell:
a: 135.783Å b: 66.644Å c: 91.971Å
α: 90° β: 109.2° γ: 90°
R-values:
R R work R free
0.193 0.191 0.218
Expression systems:
  • Escherichia coli BL21(DE3)
  • Homo sapiens

Quick links

Citations

31 review citations

Targeting cytokines to treat asthma and chronic obstructive pulmonary disease.
Barnes PJ. (2018)
30 more

9 mentions without citation

Modulation of Signaling Mediated by TSLP and IL-7 in Inflammation, Autoimmune Diseases, and Cancer.
Marković et al. (2020)
8 more