› 5j0z

X-ray diffraction
3.25Å resolution

Crystal structure of GLIC in complex with DHA

Released:
DOI: 10.2210/pdb5j0z/pdb
PDB entry 5j0z coloured by chain, front view.
PDB entry 5j0z coloured by chain, side view.
PDB entry 5j0z coloured by chain, top view.
Source organism: Gloeobacter violaceus PCC 7421
Primary publication:
Crystal structure and dynamics of a lipid-induced potential desensitized-state of a pentameric ligand-gated channel.
Basak S, Schmandt N, Gicheru Y, Chakrapani S
Elife 6 (2017)
PMID: 28262093

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo pentamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-181806 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Proton-gated ion channel Chains: A, B, C, D, E
Molecule details ›
Chains: A, B, C, D, E
Length: 311 amino acids
Theoretical weight: 35.64 KDa
Source organism: Gloeobacter violaceus PCC 7421
Expression system: Escherichia coli
UniProt:
  • Canonical: Q7NDN8 (Residues: 47-357; Coverage: 98%)
Gene names: glr4197, glvI
Sequence domains: Neurotransmitter-gated ion-channel ligand binding domain
Structure domains:

Ligands and Environments

6 bound ligands:
Ligand CL 3 x CL
Ligand LMT 6 x LMT
Ligand SO4 6 x SO4
Ligand ACT 2 x ACT
Ligand HXA 5 x HXA
Ligand PLC 1 x PLC
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: C2
Unit cell:
a: 181.868Å b: 133.32Å c: 159.919Å
α: 90° β: 102.36° γ: 90°
R-values:
R R work R free
0.233 0.232 0.261
Expression system: Escherichia coli

Quick links

Citations

6 review citations

Towards a Comprehensive Understanding of Anesthetic Mechanisms of Action: A Decade of Discovery.
Hemmings et al. (2019)
5 more

5 mentions without citation

Druggable Lipid Binding Sites in Pentameric Ligand-Gated Ion Channels and Transient Receptor Potential Channels.
Cheng et al. (2021)
4 more