› 5isa

X-ray diffraction
2.4Å resolution

Crystal structure of mouse CARM1 in complex with inhibitor SA0401

Released:
DOI: 10.2210/pdb5isa/pdb
PDB entry 5isa coloured by chain, front view.
PDB entry 5isa coloured by chain, side view.
PDB entry 5isa coloured by chain, top view.
Source organism: Mus musculus
Entry authors: Cura V, Marechal N, Mailliot J, Troffer-Charlier N, Hassenboehler P, Wurtz JM, Bonnefond L, Cavarelli J

Function and Biology Details

Reaction catalysed: 
(1a) S-adenosyl-L-methionine + [protein]-L-arginine = S-adenosyl-L-homocysteine + [protein]-N(omega)-methyl-L-arginine
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-194407 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Histone-arginine methyltransferase CARM1 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 364 amino acids
Theoretical weight: 41.24 KDa
Source organism: Mus musculus
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q9WVG6 (Residues: 130-490; Coverage: 59%)
Gene names: Carm1, Prmt4
Sequence domains: Ribosomal protein L11 methyltransferase (PrmA)
Structure domains:

Ligands and Environments

7 bound ligands:
Ligand 6D5 4 x 6D5
Ligand EDO 8 x EDO
Ligand DXE 3 x DXE
Ligand PEG 1 x PEG
Ligand M2M 1 x M2M
Ligand PG4 1 x PG4
Ligand NA 1 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-2
Spacegroup: P21212
Unit cell:
a: 74.541Å b: 98.409Å c: 205.803Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.163 0.161 0.204
Expression system: Spodoptera frugiperda

Quick links