5iqm

X-ray diffraction
1.5Å resolution

Crystal structure of the E. coli type 1 pilus subunit FimG (engineered variant with substitution Q134E; N-terminal FimG residues 1-12 truncated) in complex with the donor strand peptide DsF_T4R-T6R-D13N

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
PDBe Complex ID:
PDB-CPX-139999 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Protein FimG Chains: A, G
Molecule details ›
Chains: A, G
Length: 132 amino acids
Theoretical weight: 13.67 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P08190 (Residues: 36-167; Coverage: 92%)
Gene names: JW4282, b4319, fimG
Structure domains: Fimbrial-type adhesion domain
Protein FimF Chains: B, F
Molecule details ›
Chains: B, F
Length: 15 amino acids
Theoretical weight: 1.75 KDa
Source organism: Escherichia coli K-12
Expression system: Not provided
UniProt:
  • Canonical: P08189 (Residues: 23-37; Coverage: 10%)
Gene names: JW4281, b4318, fimF

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06DA
Spacegroup: P21
Unit cell:
a: 48.19Å b: 62.12Å c: 50.64Å
α: 90° β: 108.97° γ: 90°
R-values:
R R work R free
0.152 0.151 0.185
Expression systems:
  • Escherichia coli
  • Not provided