› 5ied

X-ray diffraction
1.81Å resolution

Murine endoplasmic reticulum alpha-glucosidase II with castanospermine

Released:
DOI: 10.2210/pdb5ied/pdb
PDB entry 5ied coloured by chain, front view.
PDB entry 5ied coloured by chain, side view.
PDB entry 5ied coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Structures of mammalian ER α-glucosidase II capture the binding modes of broad-spectrum iminosugar antivirals.
Caputo AT, Alonzi DS, Marti L, Reca IB, Kiappes JL, Struwe WB, Cross A, Basu S, Lowe ED, Darlot B, Santino A, Roversi P, Zitzmann N
Proc Natl Acad Sci U S A 113 E4630-8 (2016)
PMID: 27462106

Function and Biology Details

Reaction catalysed: 
Glc(2)Man(9)GlcNAc(2)-[protein] + H(2)O = GlcMan(9)GlcNAc(2)-[protein] + beta-D-glucopyranose
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-126675 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (3 distinct):
Neutral alpha-glucosidase AB Chain: A
Molecule details ›
Chain: A
Length: 934 amino acids
Theoretical weight: 106.16 KDa
Source organism: Mus musculus
Expression system: Homo sapiens
UniProt:
  • Canonical: Q8BHN3 (Residues: 33-187, 188-944; Coverage: 100%)
Gene names: G2an, Ganab, Kiaa0088
Sequence domains:
Glucosidase 2 subunit beta Chain: B
Molecule details ›
Chain: B
Length: 88 amino acids
Theoretical weight: 9.57 KDa
Source organism: Mus musculus
Expression system: Homo sapiens
UniProt:
  • Canonical: O08795 (Residues: 30-117; Coverage: 17%)
Gene name: Prkcsh
Sequence domains: Glucosidase II beta subunit-like

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
6 bound ligands:
Ligand PG4 4 x PG4
Ligand P6G 4 x P6G
Ligand EDO 6 x EDO
Ligand FMT 12 x FMT
Ligand CTS 1 x CTS
Ligand CA 2 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04
Spacegroup: P21212
Unit cell:
a: 103.43Å b: 173.9Å c: 63.04Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.16 0.159 0.184
Expression system: Homo sapiens

Quick links

Citations

9 review citations

Iminosugar antivirals: the therapeutic sweet spot.
Alonzi et al. (2017)
8 more

2 mentions without citation

Structures of mammalian ER α-glucosidase II capture the binding modes of broad-spectrum iminosugar antivirals.
Caputo et al. (2016)
1 more