5i8t

X-ray diffraction
1.75Å resolution

Structure of Mouse Acireductone dioxygenase with Ni2+ ion and D-lactic acid in the active site

Released:
Source organism: Mus musculus

Function and Biology Details

Reactions catalysed:
1,2-dihydroxy-5-(methylthio)pent-1-en-3-one + O(2) = 3-(methylthio)propanoate + formate + CO
1,2-dihydroxy-5-(methylthio)pent-1-en-3-one + O(2) = 4-(methylthio)-2-oxobutanoate + formate
Biochemical function:
Cellular component:

Structure analysis Details

Assembly composition:
monomeric (preferred)
PDBe Complex ID:
PDB-CPX-189331 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Acireductone dioxygenase Chain: A
Molecule details ›
Chain: A
Length: 179 amino acids
Theoretical weight: 21.55 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q99JT9 (Residues: 1-179; Coverage: 100%)
Gene names: Adi1, Mtcbp1
Sequence domains: ARD/ARD' family
Structure domains: Jelly Rolls

Ligands and Environments

3 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P41212
Unit cell:
a: 79.029Å b: 79.029Å c: 115.032Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.174 0.173 0.2
Expression system: Escherichia coli BL21(DE3)