› 5i0i

X-ray diffraction
3.15Å resolution

Crystal structure of myosin X motor domain with 2IQ motifs in pre-powerstroke state

Released:
DOI: 10.2210/pdb5i0i/pdb
PDB entry 5i0i coloured by chain, front view.
PDB entry 5i0i coloured by chain, side view.
PDB entry 5i0i coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
The myosin X motor is optimized for movement on actin bundles.
Ropars V, Yang Z, Isabet T, Blanc F, Zhou K, Lin T, Liu X, Hissier P, Samazan F, Amigues B, Yang ED, Park H, Pylypenko O, Cecchini M, Sindelar CV, Sweeney HL, Houdusse A
Nat Commun 7 12456 (2016)
PMID: 27580874

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-143909 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
Unconventional myosin-X Chains: A, B
Molecule details ›
Chains: A, B
Length: 791 amino acids
Theoretical weight: 91.55 KDa
Source organism: Homo sapiens
Expression system: unidentified baculovirus
UniProt:
  • Canonical: Q9HD67 (Residues: 3-793; Coverage: 38%)
Gene names: KIAA0799, MYO10
Sequence domains:
Calmodulin-1 Chain: C
Molecule details ›
Chain: C
Length: 145 amino acids
Theoretical weight: 16.45 KDa
Source organism: Homo sapiens
Expression system: unidentified baculovirus
UniProt:
  • Canonical: P0DP23 (Residues: 3-147; Coverage: 97%)
Gene names: CALM, CALM1, CAM, CAM1
Sequence domains: EF-hand domain pair
Calmodulin-1 Chain: E
Molecule details ›
Chain: E
Length: 145 amino acids
Theoretical weight: 16.37 KDa
Source organism: Homo sapiens
Expression system: unidentified baculovirus
UniProt:
  • Canonical: P0DP23 (Residues: 3-147; Coverage: 97%)
Gene names: CALM, CALM1, CAM, CAM1
Sequence domains: EF-hand domain pair
Calmodulin-1 Chain: G
Molecule details ›
Chain: G
Length: 43 amino acids
Theoretical weight: 4.98 KDa
Source organism: Homo sapiens
Expression system: unidentified baculovirus
UniProt:
  • Canonical: P0DP23 (Residues: 84-126; Coverage: 29%)
Gene names: CALM, CALM1, CAM, CAM1
Sequence domains: EF-hand domain pair
Calmodulin-1 Chain: I
Molecule details ›
Chain: I
Length: 64 amino acids
Theoretical weight: 7.41 KDa
Source organism: Homo sapiens
Expression system: unidentified baculovirus
UniProt:
  • Canonical: P0DP23 (Residues: 84-147; Coverage: 43%)
Gene names: CALM, CALM1, CAM, CAM1
Sequence domains: EF-hand domain pair

Ligands and Environments

5 bound ligands:
Ligand MPO 2 x MPO
Ligand MG 2 x MG
Ligand VO4 2 x VO4
Ligand ADP 2 x ADP
Ligand SO4 8 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SOLEIL BEAMLINE PROXIMA 1
Spacegroup: P212121
Unit cell:
a: 113.95Å b: 173.41Å c: 178.75Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.193 0.192 0.218
Expression system: unidentified baculovirus

Quick links

Citations

7 review citations

MyTH4-FERM myosins in the assembly and maintenance of actin-based protrusions.
Weck et al. (2017)
6 more

3 mentions without citation

Interaction of Vanadium Complexes with Proteins: Revisiting the Reported Structures in the Protein Data Bank (PDB) since 2015.
Santos et al. (2023)
2 more