› 5hx5

X-ray diffraction
2.33Å resolution

APOBEC3F Catalytic Domain Crystal Structure

Released:
DOI: 10.2210/pdb5hx5/pdb
PDB entry 5hx5 coloured by chain, front view.
PDB entry 5hx5 coloured by chain, side view.
PDB entry 5hx5 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
1.92 Angstrom Zinc-Free APOBEC3F Catalytic Domain Crystal Structure.
Shaban NM, Shi K, Li M, Aihara H, Harris RS
J Mol Biol 428 2307-2316 (2016)
PMID: 27139641

Function and Biology Details

Reaction catalysed: 
Cytosine in single-stranded DNA + H(2)O = uracil in single-stranded DNA + NH(3)
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-184861 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DNA dC->dU-editing enzyme APOBEC-3F Chains: A, B
Molecule details ›
Chains: A, B
Length: 199 amino acids
Theoretical weight: 23.36 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8IUX4 (Residues: 185-373; Coverage: 51%)
Gene name: APOBEC3F
Sequence domains: APOBEC-like N-terminal domain

Ligands and Environments

1 bound ligand:
Ligand ZN 2 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-E
Spacegroup: P21
Unit cell:
a: 35.94Å b: 50.9Å c: 100.86Å
α: 90° β: 90.75° γ: 90°
R-values:
R R work R free
0.229 0.227 0.264
Expression system: Escherichia coli

Quick links

Citations

8 review citations

APOBEC3B, a molecular driver of mutagenesis in human cancers.
Zou et al. (2017)
7 more

3 mentions without citation

APOBEC Enzymes as Targets for Virus and Cancer Therapy.
Olson et al. (2018)
2 more