› 5hso

X-ray diffraction
2.5Å resolution

Crystal structure of MYCOBACTERIUM TUBERCULOSIS MARR FAMILY PROTEIN Rv2887 complex with DNA

Released:
DOI: 10.2210/pdb5hso/pdb
PDB entry 5hso coloured by chain, front view.
PDB entry 5hso coloured by chain, side view.
PDB entry 5hso coloured by chain, top view.
Source organisms:
Primary publication:
Structural analysis of the regulatory mechanism of MarR protein Rv2887 in M. tuberculosis.
Gao YR, Li DF, Fleming J, Zhou YF, Liu Y, Deng JY, Zhou L, Zhou J, Zhu GF, Zhang XE, Wang DC, Bi LJ
Sci Rep 7 6471 (2017)
PMID: 28743871

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-118767 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
HTH-type transcriptional repressor Rv2887 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 160 amino acids
Theoretical weight: 17.01 KDa
Source organism: Mycobacterium tuberculosis H37Rv
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P9WME9 (Residues: 1-139; Coverage: 100%)
Gene names: MTCY274.18, Rv2887
Sequence domains: MarR family
DNA (30-MER), the upstream sequence of Rv0560c Chain: E
Molecule details ›
Chain: E
Length: 30 nucleotides
Theoretical weight: 9.27 KDa
Source organism: Mycobacterium tuberculosis
Expression system: Not provided
DNA (30-MER), the upstream sequence of Rv0560c Chain: F
Molecule details ›
Chain: F
Length: 30 nucleotides
Theoretical weight: 9.19 KDa
Source organism: Mycobacterium tuberculosis
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P1
Unit cell:
a: 48.218Å b: 48.233Å c: 101.115Å
α: 89.95° β: 90.04° γ: 107.13°
R-values:
R R work R free
0.241 0.24 0.278
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

3 review citations

Functional Mechanism of the Efflux Pumps Transcription Regulators From Pseudomonas aeruginosa Based on 3D Structures.
Housseini B Issa et al. (2018)
2 more

1 mention without citation

Structural analysis of the regulatory mechanism of MarR protein Rv2887 in M. tuberculosis.
Gao et al. (2017)