› 5hrb

X-ray diffraction
1.7Å resolution

The crystal structure of AsfvPolX:DNA1 binary complex

Released:
DOI: 10.2210/pdb5hrb/pdb
PDB entry 5hrb coloured by chain, front view.
PDB entry 5hrb coloured by chain, side view.
PDB entry 5hrb coloured by chain, top view.
Source organisms:
Entry authors: Chen YQ, Zhang J, Gan JH

Function and Biology Details

Reaction catalysed: 
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-118755 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
Repair DNA polymerase X Chain: A
Molecule details ›
Chain: A
Length: 178 amino acids
Theoretical weight: 20.61 KDa
Source organism: African swine fever virus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P42494 (Residues: 1-174; Coverage: 100%)
Gene names: Ba71V-97, O174L
Sequence domains:
Structure domains:
DNA (5'-D(*CP*GP*GP*AP*TP*AP*TP*CP*C)-3') Chains: D, E
Molecule details ›
Chains: D, E
Length: 9 nucleotides
Theoretical weight: 2.72 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

3 bound ligands:
Ligand MN 1 x MN
Ligand GOL 1 x GOL
Ligand BME 1 x BME
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P43
Unit cell:
a: 77.77Å b: 77.77Å c: 43.08Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.178 0.177 0.202
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

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