› 5hj9

X-ray diffraction
1.28Å resolution

Crystal structure of Leishmania mexicana arginase in complex with inhibitor ABHPE

Released:
DOI: 10.2210/pdb5hj9/pdb
PDB entry 5hj9 coloured by chain, front view.
PDB entry 5hj9 coloured by chain, side view.
PDB entry 5hj9 coloured by chain, top view.
Source organism: Leishmania mexicana
Primary publication:
Crystal structures of Leishmania mexicana arginase complexed with α,α-disubstituted boronic amino-acid inhibitors.
Hai Y, Christianson DW
Acta Crystallogr F Struct Biol Commun 72 300-6 (2016)
PMID: 27050264

Function and Biology Details

Reaction catalysed: 
L-arginine + H(2)O = L-ornithine + urea
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-180395 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Arginase Chain: A
Molecule details ›
Chain: A
Length: 330 amino acids
Theoretical weight: 36.03 KDa
Source organism: Leishmania mexicana
Expression system: Escherichia coli
UniProt:
  • Canonical: Q6TUJ5 (Residues: 13-329; Coverage: 96%)
Gene name: ARG
Sequence domains: Arginase family
Structure domains: Ureohydrolase domain

Ligands and Environments

6 bound ligands:
Ligand MN 2 x MN
Ligand X7A 1 x X7A
Ligand GOL 1 x GOL
Ligand SO4 1 x SO4
Ligand EDO 3 x EDO
Ligand PGE 2 x PGE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: R3
Unit cell:
a: 89.092Å b: 89.092Å c: 113.6Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.151 0.146 0.159
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Polyamine-trypanothione pathway: an update.
Ilari et al. (2017)
2 more

2 mentions without citation

Polyamines in protozoan pathogens.
Phillips MA. (2018)
1 more