› 5hdr

X-ray diffraction
2.3Å resolution

Crystal structure of a bacterial fucosidase with iminocyclitol (2S,3S,4R,5S)-3,4-dihydroxy-2-ethynyl-5-methylpyrrolidine

Released:
DOI: 10.2210/pdb5hdr/pdb
PDB entry 5hdr coloured by chain, front view.
PDB entry 5hdr coloured by chain, side view.
PDB entry 5hdr coloured by chain, top view.
Source organism: Bacteroides thetaiotaomicron VPI-5482
Entry authors: Wright DW, Davies GJ

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-183665 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Alpha-L-fucosidase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 447 amino acids
Theoretical weight: 51.75 KDa
Source organism: Bacteroides thetaiotaomicron VPI-5482
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8A3I4 (Residues: 35-472; Coverage: 96%)
Gene name: BT_2970
Sequence domains: Alpha-L-fucosidase
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand SO4 5 x SO4
Ligand IMD 4 x IMD
Ligand H79 4 x H79
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: P21
Unit cell:
a: 56.5Å b: 188.76Å c: 98.14Å
α: 90° β: 93.89° γ: 90°
R-values:
R R work R free
0.203 0.201 0.244
Expression system: Escherichia coli BL21(DE3)

Quick links